Data 2011 en
Data of the Consulting Laboratory for Haemophilus influenzae 2011
1. Introduction
The Consulting Laboratory for Haemophilus influenzae (KLHi) has been assigned the laboratory surveillance of invasive H. influenzae diseases in Germany by the Robert Koch Institute. Its tasks include serotyping of clinical isolates and the detection of ß-lactamase production. In 2011 submissions from 248 patients were analysed. As has been observed in previous years (e.g. 2008, cf. Robert Koch Institute, Epidemiologisches Bulletin 35/2009) the age group most affected by invasive H. influenzae disease is > 40 years (189 cases, 81.1 %).
In 234 of the submitted cases, H. influenzae was isolated from primarily sterile materials (this figure equals the number of invasive diseases).
The vast majority of the isolates consisted of non-typeable H. influenzae (NTHi, 183 cases, 78.2 %), followed by Hif as the most frequent capsular serotype (37 cases; 15.8 %). Hib showed third highest frequency among the serotypes (7 cases; 3.0 %), closely followed by Hie (6 cases, 2.6 %) and only one case of Hia (0.4 %) was detected. Serotypes c and d were not found.
The KLHi analyzed the frequency of ampicillin resistance of invasive isolates. In 2011, ampicillin E-tests were carried out in the 233 vital isolates submitted to the KLHi (in one case, only DNA was available). In 14.6 % (34 isolates) of the clinical isolates a MIC > 1 µg/ml was detected, and the majority of these strains (27 isolates, 79.4% of ampicillin-resistant strains, 11.6% of all isolates) showed ß-lactamase production.
2. Serotype distribution of invasive H. influenzae isolates
3. Age distribution of patients with invasive H. influenzae disease
4. Serotype distribution in Federal States
| BW | BY | BE | BB | HB | HH | HE | MV | NI | NW | RP | SL | SN | ST | SH | TH |
Hia | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Hib | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Hie | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Hif | 10 | 5 | 2 | 2 | 0 | 1 | 2 | 0 | 1 | 7 | 1 | 0 | 2 | 1 | 2 | 0 |
NTHi | 29 | 34 | 22 | 4 | 0 | 7 | 9 | 1 | 9 | 49 | 6 | 0 | 5 | 4 | 2 | 1 |
BW: Baden-Württemberg, BY: Bayern, BE: Berlin, BB: Brandenburg, HB: Bremen, HH: Hamburg, HE: Hessen, MV: Mecklenburg-Vorpommern, NI: Niedersachsen, NW: Nordrhein-Westfalen, RP: Rheinland-Pfalz, SL: Saarland, SN: Sachsen, ST: Sachsen-Anhalt, SH: Schleswig-Holstein, TH: Thüringen. The table comprises a total of 232 cases. One case of NTHi was an isolate from a patient living abroad. In one case, the place of residence is unknown.
5. Serotype distribution according to site of isolation
| Hia | Hib | Hie | Hif | NTHi |
Blood | 0 | 6 | 5 | 30 | 161 |
Cerebrospinal fluid | 1 | 1 | 1 | 6 | 19 |
other* | 0 | 0 | 0 | 1 | 3 |
* other materials from primarily sterile sites: cerebral abscess, joint tap
6. Ampicillin resistance in invasive H. influenzae isolates
7. Publications of the KLHi
Original Articles
- Lâm TT, Elias J, Frosch M, Vogel U, Claus H. New diagnostic PCR for Haemophilus influenzae serotype e based on the cap locus of strain ATCC 8142. Int J Med Microbiol. 2011 Feb;301(2):176-9.
- Lâm TT, Frosch M, Claus H, Vogel U. Sequence analysis of the serotype-specific synthesis regions II of Haemophilus influenzae serotypes c and d: evidence for common ancestry of capsule synthesis in Pasteurellaceae and Neisseria meningitidis. Research in Microbiology 2011 Jun;162(5):483-7.
Reviews
- Lâm TT, Claus H, Elias J, Hellenbrand W, Imöhl M, Prelog M, Sing A, van der Linden M, Vogel U. Infections with Pneumococci, Menigococci, H. influenzae and Diphtheria in Germany: the RKI Reference Network for Invasive Bacterial Infections (IBI) at the 5th Würzburg Workshop on Epidemiology, Prevention and Therapy for Invasive Meningococcal Diseases 2010 (Meeting Report). Gesundheitswesen. 2011. DOI: 10.1055/s-0031-1286269.
